| 质粒名称: | pEMTB-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000755 |
| 产品用途: | Expresses EMTB-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
EMTB, mScarlet3-H
|
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pEMTB-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEMTB-mScarlet3-H (aka mYongHong)载体质粒基本信息
pEMTB-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pEMTB-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
<img .png="" aka="" alt="pEMTB-mScarlet3-H (aka mYongHong)载体图谱 data-cke-saved-src=" uploads="" 载体图谱="" pemtb-mscarlet3-h="" (aka="" myonghong)载体图谱.png"="" src="/uploads/载体图谱/pEMTB-mScarlet3-H (aka mYongHong)载体图谱.png">
pEMTB-mScarlet3-H (aka mYongHong)质粒载体简介
pEMTB-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pEMTB-mScarlet3-H (aka mYongHong) 5522 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_755_20241014-BAN4_F03_F03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5522)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5522
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 4..592
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 767..1070
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1071..1274
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 1341..1350
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1350..1379
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1383..2186
/codon_start=1
/label=MAP7
/translation="APVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPV
LRVDDRQRLARERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEER
RRAAVEEKRRQRLEEDKERHEAVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSADPD
RRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRLQLSPWESSVVNRLLTPTHSFL
ARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSS"
regulatory 2199..2208
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2205..2894
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 3016..3137
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3144..3599)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3626..3730
/gene="bla"
/label=AmpR promoter
promoter 3732..4089
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3940..4075
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4124..4918
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5150..5197
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
61 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
661 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
721 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
781 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
841 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
901 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
961 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
1021 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
1081 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
1141 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
1201 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
1261 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct
1321 cgagctcaag cttcgaattc gccaccatgg agcagaagct catctcagaa gaagacctcg
1381 gcgcgccagt gcgaagcgaa acagcacccg acagctacaa agtgcaagat aagaaaaatg
1441 cctccagccg ccctgcctct gcaatttcag gacaaaataa caaccactca ggaaataaac
1501 cagaccctcc gcctgtgtta cgtgttgatg accggcagcg gctggcccgg gagcgacgtg
1561 aggaacggga gaaacagcta gctgcaagag aaatagtgtg gttagaaaga gaagagcgag
1621 ccaggcagca ctacgagaag cacctggaag agcggaagaa gaggttggag gagcagaggc
1681 agaaggagga gcggaggagg gctgctgtgg aggagaagcg gaggcagaga cttgaggagg
1741 acaaagaacg ccacgaagct gttgtacggc gcacaatgga aaggagccag aagccaaaac
1801 agaagcataa ccgttggtcg tggggaggct ctctccatgg gagccctagc atccacagtg
1861 cagatccaga caggcggtca gtttccacca tgaatctttc gaaatatgtt gatcccgtca
1921 ttagcaagcg gctctcctct tcatctgcaa ctttactaaa ttctccagat agagctcgcc
1981 gcctgcagct cagcccatgg gagagcagcg ttgttaacag actcctgacg cccacacatt
2041 cgttcctggc cagaagtaaa agcacagctg ccttgtctgg agaagcagca tcttgcagcc
2101 ccatcatcat gccctacaaa gctgcacact ctagaaattc gatggatcga ccaaaactct
2161 ttgtaacacc acctgagggc tcttcggatc caccggtcgc caccatggat agcaccgagg
2221 cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg aacggccacg
2281 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
2341 tgagggtgac caagggtggc cccctgccct tctcctggga catcctgtcc cctcagttca
2401 tgtacggctc cagggccttc acgaagcacc ccgccgacat ccccgactac tggaagcagt
2461 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgccgtgt
2521 ccgtggccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctccgcg
2581 gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc tgggaagcat
2641 ccaccgagcg gttgtacccc gaggacgtcg tgctgaaggg cgacattaag catgccctgc
2701 gcctgaagga cggcggccgg tacctggcgg acttcaagac cacctacagg gccaagaagc
2761 ccgtgcagat gcccggcgcc ttcaacatcg accgcaagtt ggacatcaca tcccacaacg
2821 aggactacac cgtggtggaa cagtacgaac gctccgtggc ccgccactcc accggcggct
2881 ccggtggctc ctaagcggcc gcgactctag atcataatca gccataccac atttgtagag
2941 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
3001 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
3061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
3121 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
3181 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
3241 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
3301 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
3361 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
3421 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
3481 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
3541 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
3721 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
3841 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3901 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
3961 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
4021 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
4081 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
4141 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
4201 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
4261 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
4321 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
4381 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
4441 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
4501 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
4561 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
4621 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
4681 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
4741 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
4801 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
4861 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
4921 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
4981 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
5041 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
5101 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
5161 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
5221 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
5281 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
5341 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
5401 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
5461 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5521 tc
//
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